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BIOTECHNOLOGY PRINCIPLES AND PROCESSES
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BIOTECHNOLOGY PRINCIPLES AND PROCESSES
Very Short Answer Questions
Q1. Define biotechnology.
Q2. What are molecular scissors? Where are they obtained from?
Q3. Name any two artificially restructured plasmids.
Q4. What is EcoR1? How does it function?
Q5. What are cloning vectors? Give an example.
Q6. What is recombinant DNA?
Q7. What is palindromic sequence?
Q8. What is the full form of PCR? How is it useful in biotechnology?
Q9. What is processing? down-stream
Q10. How does one visualize DNA on an agar gel.
Q11. How can you differentiate between exonucleases and endonucleases?
Q1. Write short notes on restriction enzymes.
Q2. Give an account of amplification of gene of interest using PCR.
Q3. What is a bio-reactor? Describe briefly the stirring type of bio- reactor.
Q4. What are the different methods of insertion of recombinant DNA into the host cell?
Q1. Explain briefly the various processes of recombinant DNA technology.
Q2. Give a brief account of the tools of recombinant DNA technology.
Q1. Do eukaryotic cells have restriction endonucleases? Justify your answer.
Q2. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Q3. Can you recall meiosis and Indicate at what stage a recombinant DNA is made?
Q4. Describe briefly the following:(a) Origin of replication(b) Bioreactors(c) Downstream processing
Q5. Explain briefly(a) PCR(b) Restriction enzymes and DNA(c) Chitinase
Q6. Discuss with your teacher and find out how to distinguish between(a) Plasmid DNA Chromosomal DNA and(b) RNA and DNA(c) Exonuclease Endonuclease and
Q7. What does 'H' 'in' 'd' and 'III' refer to in the enzyme Hind III?
Q8. Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Q9. What does 'competent' refer to in 'competent cells' used in transformation experiments?
Q10. What is the significance of adding proteases at the time of isolation of genetic material (DNA).
Q11. While doing a PCR, 'denaturation' step is missed. What will be its effect on the process?
Q12. What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Q13. What is meant by gene cloning?
Q14. Decide the ratio between ester bonds and hydrogen bonds that are broken in each palindromic sequence of a DNA when treated with EcoRI during the formation of sticky ends.